In-silico driven engineering of enantioselectivity of a penicillin G acylase towards active pharmaceutical ingredients

0483930 - MBÚ 2018 RIV NL eng J - Článek v odborném periodiku
Grulich, Michal - Brezovský, J. - Štěpánek, Václav - Palyzová, Andrea - Marešová, Helena - Zahradník, Jiří - Kyslíková, Eva - Kyslík, Pavel
In-silico driven engineering of enantioselectivity of a penicillin G acylase towards active pharmaceutical ingredients.
Journal of Molecular Catalysis B-Enzymatic. Roč. 133, Supplement 1 (2016), s. 53-59. ISSN 1381-1177
Institucionální podpora: RVO:61388971
Klíčová slova: Docking experiments * Enantioselectivity * Penicillin G acylase
Obor OECD: Microbiology
Impakt faktor: 2.269, rok: 2016

Penicillin G acylase is one of the most employed enzymes in the pharmaceutical industry due to its role in the biotransformation of semi-synthetic Beta-lactam antibiotics. Recently, the enantioselectivity of the penicillin G acylase markedly broadened its application potential. In this study, we have evaluated effects of in-silico replacements of acyl-binding subsite residue Phe24Beta of the enzyme from Achromobacter sp. CCM 4824 to seven markedly smaller amino acids on its enantioselectivity towards industrially relevant compounds. Models of the two most promising mutants bearing substitutions PheBeta24Ala and PheBeta24Cys were investigated using molecular docking calculations. The Cys substitution revealed much better enantioselectivity traits with a set of seven substrates. To verify the relevance of in-silico predictions, we constructed a PGA. A + Phe24BetaCys mutant and determined its enantioselectivity in biocatalytic reactions. Since we experimentally confirmed all these predictions, we expanded our in-silico analysis to another set of seven compounds: the prediction suggested increased enantioselectivity for N-phenylacetyl-p-F-alfa-phenylalanine. The (R)-enantiomer of this substrate is used as a building block in synthesis of important anti-cancer agent Abarelix. The enantioselectivity of PGA. A + Phe24BetaCys mutant towards this substrate was improved by 75percent reaching E-value of about 70. Our results suggest the rapid identification of interesting replacements altering enantioselectivity using in-silico approach as the way for further expanding biotechnological application of penicillin G acylase
Trvalý link: http://hdl.handle.net/11104/0279725