Secondary Metabolites Produced during the Germination of Streptomyces coelicolor

0483716 - MBÚ 2018 RIV CH eng J - Článek v odborném periodiku
Čihák, M. - Kameník, Zdeněk - Šmídová, Klára - Bergman, N. - Benada, Oldřich - Kofroňová, Olga - Petříčková, Kateřina - Bobek, Jan
Secondary Metabolites Produced during the Germination of Streptomyces coelicolor.
Frontiers in Microbiology. Roč. 8, DEC 13 (2017), č. článku 2495. ISSN 1664-302X. E-ISSN 1664-302X
Grant CEP: GA MŠMT(CZ) LO1509; GA MŠMT(CZ) LM2015055
Institucionální podpora: RVO:61388971
Klíčová slova: spore germination * Streptomyces * cell signaling
Obor OECD: Microbiology
Impakt faktor: 4.019, rok: 2017

Spore awakening is a series of actions that starts with purely physical processes and continues via the launching of gene expression and metabolic activities, eventually achieving a vegetative phase of growth. In spore-forming microorganisms, the germination process is controlled by intra-and inter-species communication. However, in the Streptomyces clade, which is capable of developing a plethora of valuable compounds, the chemical signals produced during germination have not been systematically studied before. Our previously published data revealed that several secondary metabolite biosynthetic genes are expressed during germination. Therefore, we focus here on the secondary metabolite production during this developmental stage. Using high-performance liquid chromatography-mass spectrometry, we found that the sesquiterpenoid antibiotic albaflavenone, the polyketide germicidin A, and chalcone are produced during germination of the model streptomycete, S. coelicolor. Interestingly, the last two compounds revealed an inhibitory effect on the germination process. The secondary metabolites originating from the early stage of microbial growth may coordinate the development of the producer (quorum sensing) and/or play a role in competitive microflora repression (quorum quenching) in their nature environments.
Trvalý link: http://hdl.handle.net/11104/0278919