Transmembrane helix connectivity in Orai1 controls two gates for calcium-dependent transcription

0483350 - MBÚ 2018 RIV US eng J - Článek v odborném periodiku
Frischauf, I. - Litviňuková, M. - Schober, R. - Zayats, Vasilina - Svobodová, B. - Bonhenry, Daniel - Lunz, V. - Cappello, S. - Tociu, L. - Řeha, David - Stallinger, A. - Hochreiter, A. - Pammer, T. - Butorac, C. - Muik, M. - Groschner, K. - Bogeski, I. - Ettrich, Horst Rüdiger - Romanin, Ch. - Schindl, R.
Transmembrane helix connectivity in Orai1 controls two gates for calcium-dependent transcription.
Science Signaling. Roč. 10, č. 507 (2017), č. článku eaao0358. ISSN 1945-0877. E-ISSN 1937-9145
Grant CEP: GA ČR(CZ) GA13-21053S; GA MŠMT(CZ) LM2015055; GA MŠMT(CZ) LTC17069
Institucionální podpora: RVO:61388971
Klíčová slova: COMPREHENSIVE MOLECULAR CHARACTERIZATION * FAST CA2+-DEPENDENT INACTIVATION * ACTIVATES CRAC CHANNELS
Obor OECD: Biochemistry and molecular biology
Impakt faktor: 6.378, rok: 2017

The mutation of Orai1 channel resulting in constitutively active gating were investigated. Analysis of the constitutively open Orai1 mutant channels revealed two fundamental gates that enabled Ca2+ influx: Arginine side chains were displaced so they no longer blocked the pore, and a chain of water molecules formed in the hydrophobic pore region. Together, these results enabled us to identify a cluster of Orai1 mutations that trigger Ca2+ permeation associated with gene transcription and provide a gating mechanism for Orai1.
Trvalý link: http://hdl.handle.net/11104/0278714