Anchored but not internalized: shape dependent endocytosis of nanodiamond

0483228 - ÚOCHB 2018 RIV GB eng J - Článek v odborném periodiku
Zhang, B. - Feng, X. - Yin, H. - Ge, Z. - Wang, Y. H. - Chu, Z. - Raabová, Helena - Vávra, Jan - Cígler, Petr - Liu, R. - Wang, Y. - Li, Q.
Anchored but not internalized: shape dependent endocytosis of nanodiamond.
Scientific Reports. Roč. 7, Apr 13 (2017), č. článku 46462. ISSN 2045-2322. E-ISSN 2045-2322
Grant CEP: GA MZd(CZ) NV15-33094A; GA MŠMT(CZ) 7AMB16FR029
Institucionální podpora: RVO:61388963
Klíčová slova: fluorescent nanodiamonds * surface chemistry * vesicle membranes
Obor OECD: Cell biology
Impakt faktor: 4.122, rok: 2017
https://www.nature.com/articles/srep46462

Nanoparticle-cell interactions begin with the cellular uptake of the nanoparticles, a process that eventually determines their cellular fate. In the present work, we show that the morphological features of nanodiamonds (NDs) affect both the anchoring and internalization stages of their endocytosis. While a prickly ND (with sharp edges/corners) has no trouble of anchoring onto the plasma membrane, it suffers from difficult internalization afterwards. In comparison, the internalization of a round ND (obtained by selective etching of the prickly ND) is not limited by its lower anchoring amount and presents a much higher endocytosis amount. Molecular dynamics simulation and continuum modelling results suggest that the observed difference in the anchoring of round and prickly NDs likely results from the reduced contact surface area with the cell membrane of the former, while the energy penalty associated with membrane curvature generation, which is lower for a round ND, may explain its higher probability of the subsequent internalization.
Trvalý link: http://hdl.handle.net/11104/0278615