Broad-range survey of vector-borne pathogens and tick host identification of Ixodes ricinus from Southern Czech Republic

0482910 - BC 2018 RIV GB eng J - Článek v odborném periodiku
Hönig, Václav - Carolan, H. E. - Vavrušková, Zuzana - Massire, C. - Mosel, M.l R. - Crowder, C. D. - Rounds, M. A. - Ecker, D. J. - Růžek, Daniel - Grubhoffer, Libor - Luft, B. J. - Eshoo, M. W.
Broad-range survey of vector-borne pathogens and tick host identification of Ixodes ricinus from Southern Czech Republic.
FEMS Microbiology Ecology. Roč. 93, č. 11 (2017), č. článku fix129. ISSN 0168-6496. E-ISSN 1574-6941
GRANT EU: European Commission(XE) 278976 - ANTIGONE
Institucionální podpora: RVO:60077344
Klíčová slova: burgdorferi sensu-lato * tick * Ixodes ricinus * pcr-esi/ms * Borrelia * host * Lyme borreliosis * Babesia * Anaplasma * Rickettsia
Obor OECD: Microbiology
Impakt faktor: 3.495, rok: 2017

Ixodes ricinus ticks are vectors of numerous human and animal pathogens. They are host generalists able to feed on more than 300 vertebrate species. The prevalence of tick-borne pathogens is influenced by host-vector-pathogen interactions that results in spatial distribution of infection risk. Broad-range polymerase chain reaction electrospray ionization mass spectrometry (PCR/ESI-MS) was used to analyze 435 I. ricinus nymphs from four localities in the south of the Czech Republic for the species identification of tick-borne pathogens. Borrelia burgdorferi sensu lato spirochetes were the most common pathogen detected in the ticks, 21% of ticks were positive for a single genospecies and 2% were co-infected with two genospecies. Other tick-borne pathogens detected included Rickettsia helvetica (3.9%), R. monacensis (0.2%), Anaplasma phagocytophilum (2.8%), Babesia venatorum (0.9%), and Ba. microti (0.5%). The vertebrate host of the ticks was determined using PCR followed by reverse line blot hybridization from the tick's blood-meal remnants. The host was identified for 61% of ticks. DNA of two hosts was detected in 16% of samples with successful host identification. The majority of ticks had fed on artiodactyls (50.7%) followed by rodents (28.6%) and birds (7.8%). Other host species were wild boar, deer, squirrels, field mice and voles.
Trvalý link: http://hdl.handle.net/11104/0278303