The use of real time PCR method for evaluation of magnetic microspheres

0482440 - ÚMCH 2018 CZ eng C - Konferenční příspěvek (zahraniční konf.)
Trachtová, Š. - Rittich, B. - Horák, Daniel - Španová, A.
The use of real time PCR method for evaluation of magnetic microspheres.
CECE 2012. 9th International Interdisciplinary Meeting on Bioanalysis. Brno: Ústav analytické chemie AV ČR, v. v. i, 2012 - (Foret, F.; Křenková, J.; Guttman, A.; Klepárník, K.; Boček, P.), s. 374-378. ISBN 978-80-904959-1-3.
[CECE 2012. International Interdisciplinary Meeting on Bioanalysis /9./. Brno (CZ), 01.11.2012-02.11.2012]
Institucionální podpora: RVO:61389013
Klíčová slova: magnetic microspheres * real time PCR
Kód oboru RIV: CD - Makromolekulární chemie

The real-time quantitative PCR (qPCR) was used for the evaluation of non-porous HEMA-based magnetic microspheres on the PCR course (PCR sensitivity). It was shown that 1-10 mu l microsphere suspensions (2 mg/ml) in PCR mixtures do not interfere in the PCR. It can be assumed that the magnetic nuclei were covered with polymer layer.
Trvalý link: http://hdl.handle.net/11104/0279473