Effects of Infrared Optical Trapping on Saccharomyces cerevisiae in a Microfluidic System

0481578 - ÚPT 2018 RIV CH eng J - Článek v odborném periodiku
Pilát, Zdeněk - Jonáš, A. - Ježek, Jan - Zemánek, Pavel
Effects of Infrared Optical Trapping on Saccharomyces cerevisiae in a Microfluidic System.
Sensors. Roč. 17, NOV (2017), s. 1-12, č. článku 2640. E-ISSN 1424-8220
Grant CEP: GA MŠMT(CZ) LO1212; GA MŠMT ED0017/01/01
Institucionální podpora: RVO:68081731
Klíčová slova: optical trapping * microfluidics * phototoxicity * laser * Saccharomyces cerevisiae
Obor OECD: Optics (including laser optics and quantum optics)
Impakt faktor: 2.475, rok: 2017
http://www.mdpi.com/1424-8220/17/11/2640

Baker’s yeast (Saccharomyces cerevisiae) represents a very popular single-celled eukaryotic model organism which has been studied extensively by various methods and whose genome has been completely sequenced. It was also among the first living organisms that were manipulated by optical tweezers and it is currently a frequent subject of optical micromanipulation experiments. We built a microfluidic system for optical trapping experiments with individual cells and used it for the assessment of cell tolerance to phototoxic stress. Using optical tweezers with the wavelength of 1064 nm, we trapped individual Saccharomyces cerevisiae cells for 15 min and, subsequently, observed
their stress response in specially designed microfluidic chambers over time periods of several hours by time-lapse video microscopy. We determined the time between successive bud formations after the exposure to the trapping light, took account of damaged cells, and calculated the population doubling period and cell areas for increasing trapping power at a constant trapping time. Our approach represents an attractive, versatile microfluidic platform for quantitative optical trapping experiments
with living cells. We demonstrate its application potential by assessing the limits for safe, non-invasive optical trapping of Saccharomyces cerevisiae with infrared laser light.
Trvalý link: http://hdl.handle.net/11104/0277110