Miniaturized device for single-cell analysis of Caspase-3 and 7 activation using bioluminiscence chemistry

0481537 - ÚIACH 2018 RIV HU eng C - Konferenční příspěvek (zahraniční konf.)
Ledvina, Vojtěch - Klepárník, Karel
Miniaturized device for single-cell analysis of Caspase-3 and 7 activation using bioluminiscence chemistry.
CECE 2017. Veszprém: University of Pannonia, 2017 - (Guttman, A.; Lindner, H.; Járvás, G.; Hajba, L.; Mészáros, B.), s. 69-72. ISBN 978-963-396-103-2.
[International Interdisciplinary Meeting on Bioanalysis /14./. Veszprém (HU), 08.10.2017-11.10.2017]
Grant CEP: GA ČR(CZ) GA17-01995S
Institucionální podpora: RVO:68081715
Klíčová slova: bioluminescence * single-cell analysis * caspases
Obor OECD: Analytical chemistry

A miniaturized device was developed for the analysis of caspase activation in single cells using the bioluminescence chemistry based on modified aminoluciferin-firefly luciferase system. A limit of detection below the average content of caspase-3 and 7 in single cells was reached.
Trvalý link: http://hdl.handle.net/11104/0277073