Metabolism of ibuprofen in higher plants: A model Arabidopsis thaliana cell suspension culture system

0481319 - ÚEB 2018 RIV GB eng J - Článek v odborném periodiku
Maršík, Petr - Šíša, Miroslav - Lacina, O. - Moťková, Kateřina - Langhansová, Lenka - Rezek, Jan - Vaněk, Tomáš
Metabolism of ibuprofen in higher plants: A model Arabidopsis thaliana cell suspension culture system.
Environmental Pollution. Roč. 220, JAN (2017), s. 383-392. ISSN 0269-7491. E-ISSN 1873-6424
Grant CEP: GA ČR(CZ) GA14-22593S
Grant ostatní: European Regional Development Fund(XE) CZ.2.16/3.1.00/24014
Institucionální podpora: RVO:61389030
Klíčová slova: Arabidopsis thaliana * Ibuprofen * Metabolism * Plant cells * Sequestration
Obor OECD: Plant sciences, botany
Impakt faktor: 4.358, rok: 2017

The uptake and metabolism of ibuprofen (IBU) by plants at the cellular level was investigated using a suspension culture of A. thaliana. Almost all IBU added to the medium (200 μM) was metabolized or bound to insoluble structures in 5 days. More than 300 metabolites were determined by liquid chromatography-high resolution mass spectrometry (LC-HRMS) analysis, and most of these are first reported for plants here. Although hydroxylated derivatives formed by oxidation on the isobutyl side chain were the main first-step products of IBU degradation, conjugates of these products with sugar, methyl and amino acid groups were the dominant metabolites in the culture. The main portion of total added IBU (81%) was accumulated in the extractable intracellular pool, whereas the cultivation medium fraction contained only 19%. The amount of the insoluble cell-wall-bound IBU was negligible (0.005% of total IBU).
Trvalý link: http://hdl.handle.net/11104/0276900