Real-time monitoring of stem cells by diamond-based impedance sensors

0480303 - FZÚ 2018 RIV CH eng C - Konferenční příspěvek (zahraniční konf.)
Procházka, Václav - Matějka, Roman - Ižák, Tibor - Szabó, Ondrej - Štěpanovská, J. - Bačáková, Lucie - Kromka, Alexander
Real-time monitoring of stem cells by diamond-based impedance sensors.
Proceedings (Basel, Switzerland). Vol. 1. Basel: MDPI, 2017, s. 1-4, č. článku 515. E-ISSN 2504-3900.
[Eurosensors 2017. Paris (FR), 03.09.2017-06.09.2017]
Grant CEP: GA MZd(CZ) NV15-33018A
Institucionální podpora: RVO:68378271 ; RVO:67985823
Klíčová slova: diamond film * biosensor * stem cells * impedance spectroscopy
Obor OECD: Electrical and electronic engineering; Electrical and electronic engineering (FGU-C)

Cell-based impedance spectroscopy is a promising label-free method for electrical monitoring of cell activity. Here we present a diamond-based impedance sensor with built-in gold interdigitated electrodes (IDEs) as a promising platform for simultaneous electrical and optical monitoring of adipose-derived stem cells (ASC). The impedance spectra were collected in a wide frequency range (from 100 Hz to 50 kHz) for 27 h of cultivation in chambers designed for static cultivation. Absolute impedance spectra were analyzed in terms of measurement frequencies and cell properties monitored by high-resolution digital camera.
Trvalý link: http://hdl.handle.net/11104/0276121