Remodeling of tick cytoskeleton in response to infection with Anaplasma phagocytophilum

0478939 - BC 2018 RIV US eng J - Článek v odborném periodiku
Cabezas Cruz, Alejandro - Alberdi, P. - Valdés, James J. - Villar, M. - de la Fuente, J.
Remodeling of tick cytoskeleton in response to infection with Anaplasma phagocytophilum.
Frontiers in Bioscience-Landmark. Roč. 22, Jun 1 (2017), s. 1830-1844. ISSN 1093-4715
GRANT EU: European Commission(XE) 278976 - ANTIGONE
Institucionální podpora: RVO:60077344
Klíčová slova: cytoskeleton * proteomics * transcriptomics * Ixodes scapularis * Anaplasma phagocytophilum
Obor OECD: Biochemistry and molecular biology
Způsob publikování: Omezený přístup
https://www.bioscience.org/2017/v22/af/4574/list.htm

The obligate intracellular pathogen Anaplasma phagocytophilum infects vertebrate and tick hosts. In this study, a genome-wide search for cytoskeleton components was performed in the tick vector, Ixodes scapularis. The available transcriptomics and proteomics data was then used to characterize the mRNA and protein levels of I. scapularis cytoskeleton components in response to A. phagocytophilum infection. The results showed that cytoskeleton components described in other model organisms were present in the I. scapularis genome. One type of intermediate filaments (lamin), a family of septins that was recently implicated in the cellular response to intracellular pathogens, and several members of motor proteins (kinesins and dyneins) that could be implicated in the cytoplasmic movements of A. phagocytophilum were found. The results showed that levels of tubulin, actin, septin, actin-related proteins and motor proteins were affected by A. phagocytophilum, probably to facilitate infection in I. scapularis. Functional studies demonstrated a role for selected cytoskeleton components in pathogen infection. These results provided a more comprehensive view of the cytoskeletal components involved in the response to A. phagocytophilum infection in ticks.
Trvalý link: http://hdl.handle.net/11104/0274985