Analysis of proteomes released from in vitro cultured eight Clostridium difficile PCR ribotypes revealed specific expression in PCR ribotypes 027 and 176 confirming their genetic relatedness and clinical importance at the proteomic level

0478190 - ÚOCHB 2018 RIV GB eng J - Článek v odborném periodiku
Dresler, J. - Krůtová, M. - Fučíková, A. - Klimentová, J. - Hrůzová, V. - Ďuráčová, M. - Houdková, K. - Salovská, B. - Matějková, J. - Hubálek, Martin - Pajer, P. - Píša, L. - Nyč, O.
Analysis of proteomes released from in vitro cultured eight Clostridium difficile PCR ribotypes revealed specific expression in PCR ribotypes 027 and 176 confirming their genetic relatedness and clinical importance at the proteomic level.
Gut Pathogens. Roč. 9, Aug 14 (2017), č. článku 45. ISSN 1757-4749. E-ISSN 1757-4749
Institucionální podpora: RVO:61388963
Klíčová slova: Clostridium difficile * label-free quantification * proteome * PCR ribotype 027 * PCR ribotype 176
Obor OECD: Microbiology
Impakt faktor: 2.809, rok: 2017
https://gutpathogens.biomedcentral.com/articles/10.1186/s13099-017-0194-9

Clostridium difficile is the causative agent of C. difficile infection (CDI) that could be manifested by diarrhea, pseudomembranous colitis or life-threatening toxic megacolon. The spread of certain strains represents a significant economic burden for health-care. The epidemic successful strains are also associated with severe clinical features of CDI. Therefore, a proteomic study has been conducted that comprises proteomes released from in vitro cultured panel of eight different PCR ribotypes (RTs) and employs the combination of shotgun proteomics and label-free quantification (LFQ) approach. The comparative semi-quantitative analyses enabled investigation of a total of 662 proteins. Both hierarchical clustering and principal component analysis (PCA) created eight distinctive groups. From these quantifiable proteins, 27 were significantly increased in functional annotations. Among them, several known factors connected with virulence were identified, such as toxin A, B, binary toxin, flagellar proteins, and proteins associated with Pro-Pro endopeptidase (PPEP-1) functional complex. Comparative analysis of protein expression showed a higher expression or unique expression of proteins linked to pathogenicity or iron metabolism in RTs 027 and 176 supporting their genetic relatedness and clinical importance at the proteomic level. Moreover, the absence of putative nitroreductase and the abundance of the Abc-type fe3+ transport system protein were observed as biomarkers for the RTs possessing binary toxin genes (027, 176 and 078). Higher expression of selected flagellar proteins clearly distinguished RTs 027, 176, 005 and 012, confirming the pathogenic role of the assembly in CDI. Finally, the histidine synthesis pathway regulating protein complex HisG/HisZ was observed only in isolates possessing the genes for toxin A and B.
Trvalý link: http://hdl.handle.net/11104/0274367