Počet záznamů: 1  

Cholesteryl esters of omega-(O-acyl)-hydroxy fatty acids in vernix caseosa

  1. 1.
    0478141 - ÚOCHB 2018 RIV US eng J - Článek v odborném periodiku
    Kalužíková, Aneta - Vrkoslav, Vladimír - Harazim, Eva - Hoskovec, Michal - Plavka, R. - Buděšínský, Miloš - Bosáková, Z. - Cvačka, Josef
    Cholesteryl esters of omega-(O-acyl)-hydroxy fatty acids in vernix caseosa.
    Journal of Lipid Research. Roč. 58, č. 8 (2017), s. 1579-1590. ISSN 0022-2275. E-ISSN 1539-7262
    Grant CEP: GA ČR GAP206/12/0750
    Institucionální podpora: RVO:61388963
    Klíčová slova: skin lipids * neutral lipids * cholesterol * lipidomics * mass spectrometry
    Obor OECD: Analytical chemistry
    Impakt faktor: 4.505, rok: 2017

    Cholesteryl esters of omega-(O-acyl)-hydroxy FAs (Chl. OAHFAs) were identified for the first time in vernix caseosa and characterized using chromatography and MS. Chl-omega OAHFAs were isolated using adsorption chromatography on silica gel and magnesium hydroxide. Their general structure was established using high-resolution and tandem MS of intact lipids, and products of their transesterification and derivatizations. Individual molecular species were characterized using nonaqueous reversed-phase HPLC coupled to atmospheric pressure chemical ionization. The analytes were detected as protonated molecules, and their structures were elucidated in the negative ion mode using controlled thermal decomposition and data-dependent fragmentation. About three hundred molecular species of Chl-omega OAHFAs were identified in this way. The most abundant Chl. OAHFAs contained 32:1 omega-hydroxy FA (omega-HFA) and 14:0, 15:0, 16:0, 16:1, and 18:1 FAs. The double bond in the 32:1 omega-HFA was in the n-7 and n-9 positions. Chl-omega OAHFAs are estimated to account for approximately 1-2% of vernix caseosa lipids.
    Trvalý link: http://hdl.handle.net/11104/0274346

     
     
Počet záznamů: 1  

  Tyto stránky využívají soubory cookies, které usnadňují jejich prohlížení. Další informace o tom jak používáme cookies.