Bifidobacterium longum CCM 7952 prevents acute DSS-induced colitis in stricly strain-specific manner

0478081 - MBÚ 2018 eng A - Abstrakt
Šrůtková, Dagmar - Schwarzer, Martin - Hudcovic, Tomáš - Jirásková Zákostelská, Zuzana - Španová, A. - Rittich, B. - Schabussová, I. - Kozáková, Hana
Bifidobacterium longum CCM 7952 prevents acute DSS-induced colitis in stricly strain-specific manner.
Food, Microbiota and Immunity „For strong immunity – feed your microbiota well“. Praha: Czech immunological society, 2017. s. 54-55.
[Food, Microbiota and Immunity. 07.06.2017-10.06.2017, Třešť]
Grant CEP: GA MŠMT(CZ) 7AMB16PL006
Institucionální podpora: RVO:61388971
Klíčová slova: Bifidobacterium longum * inflammatory bowel disease * colitis
Obor OECD: Microbiology

Administration of probiotics has been suggested as a promising strategy for prevention of inflammatory bowel diseases. Ten bifidobacterial isolates from the stool of healthy individuals were chosen for their good survival in low pH and cholic salts milieu. Formalin inactivated bacterial strains were cultivated with murine spleen cells. We found that bifidobacteria affect the immune response of spleen cells to varying intensity. Strain B. longum 7952 compared to strain 372 stimulated lower levels of IFN-gamma, TNF-alpha and IL-10 in naïve splenocytes or DC; also the induction of co-stimulatory molecules on DC was less pronounced. Experimental model of ulcerative colitis was used for in vivo study of potential anti-inflammatory effect of these bifidobacterial strains. In DSS-model, B. longum 7952 strain reduced macroscopic and histological signs of intestinal inflammation and downregulated the level of pro-inflammatory cytokines in MLN compared to the strain 372 or PBS-control. On the other hand, immunohistochemical evaluation of ZO-1 and occludin showed elevated amount of these proteins in colon descendens of mice pretreated by the strain 7952. In this respect, the strain 7952 improved intestinal barrier function as demonstrated by reduced FITC-dextran levels in serum. We showed that immunomodulatory properties of bifidobacteria are strain-dependent and careful selection might be crucial in providing beneficial outcome in clinical trials with probiotics in IBD
Trvalý link: http://hdl.handle.net/11104/0274369