Microbial colonization as driving factor in intestinal lymphocyte development

0478077 - MBÚ 2018 eng A - Abstrakt
Šinkora, Marek - Butler, J. E. - David, F.
Microbial colonization as driving factor in intestinal lymphocyte development.
Food, Microbiota and Immunity „For strong immunity – feed your microbiota well“. Praha: Czech immunological society, 2017. s. 14-14.
[Food, Microbiota and Immunity. 07.06.2017-10.06.2017, Třešť]
Grant CEP: GA ČR GA15-02274S; GA ČR(CZ) GA16-09296S
Institucionální podpora: RVO:61388971
Klíčová slova: immunity of gut * microbial colonization * ileal Payer’s patches
Obor OECD: Microbiology

Comparison of ileum-resected, surgical shams and untreated germ-free piglets, all of which were later colonized with a defined commensal flora, demonstrated that resection of the ileum did not alter the level and phenotype of B and T cells in lymphoid tissues and the blood 10 weeks after surgery. Additionally, colonization caused a shift from the fetal type of lymphocyte distribution to the adult type that is characterized by prevalence of B cells, with many of them representing class-switched, primed and antibody-producing or memory cells. Moreover, colonization leads to appearance of effector and memory T cells. Comparison of germ-free with colonized pigs indicated that lymphocyte development in the gut is dependent on colonization. These results indicate that ileum is not required for systemic B cell generation, diversification or maintenance, but it is a secondary lymphoid tissue that appears important in immune responses to colonizing bacteria
Trvalý link: http://hdl.handle.net/11104/0274277