Počet záznamů: 1  

Influence of gut microbiota on psychosocial stress and consequence in peripheral tissues of gnotobiotic mice

  1. 1.
    0478016 - MBÚ 2018 CZ eng A - Abstrakt
    Hudcovic, Tomáš - Hermanová, Petra - Šestáková, Blanka - Hajná, Vladimíra - Vodička, Martin - Ergang, Peter - Mikulecká, Anna - Kozáková, Hana - Pácha, Jiří
    Influence of gut microbiota on psychosocial stress and consequence in peripheral tissues of gnotobiotic mice.
    Food, Microbiota and Immunity „For strong immunity – feed your microbiota well“. Praha: Czech immunological society, 2017. s. 48-48.
    [Food, Microbiota and Immunity. 07.06.2017-10.06.2017, Třešť]
    Grant CEP: GA ČR GA15-07268S
    Institucionální podpora: RVO:61388971
    Klíčová slova: psychosocial stress * germ-free and specific pathogen free mice * neurohormone and cytokine
    Obor OECD: Microbiology

    The role of gut microbiota in chronic psychosocial stress was studied using germ-free (GF) and specific pathogen free (SPF) males of BALB/c mice. Stress was elicited using resident-intruder paradigm where young male was placed in a cage of an unfamiliar male. After 10 min of free interaction, mice were separated by a steel grate, for following 50 min. This procedure was repeated in five days and interactions were video-recorded. The changes in behavior during social interactions and the levels of neurohormone and cytokine mRNAs were investigated. We have found reduced anxiety in GF males, compared to SPF counterparts. Stress increased the expression of adrenal tyrosinhydroxylase and phenyletanolamin-N-metyltransferase and this effect was significantly more pronounced in GF than SPF. No effect of gut microbiota was found in expression of proopiomelanocortin in pituitary; however in SPF we found higher mRNA expression of glucocorticoid receptor (GR), than in GF. Chronic stress also led to slight decrease of GR in SPF mice. Stress decreased splenic IL-4, IL-6, IL-10 and IFN in both GF and SPF whereas TNFa and IL-1b only in GF. We conclude that microbiota impact anxiety, stress and immune response to psychosocial stress
    Trvalý link: http://hdl.handle.net/11104/0274259

     
     
Počet záznamů: 1  

  Tyto stránky využívají soubory cookies, které usnadňují jejich prohlížení. Další informace o tom jak používáme cookies.