Needleless emulsion electrospinning for the regulated delivery of susceptible proteins.

0477266 - ÚEM 2019 RIV GB eng J - Článek v odborném periodiku
Buzgo, Matej - Filová, Eva - Staffa, Andrea - Rampichová, Michala - Doupnik, M. - Vocetková, Karolína - Lukášová, Věra - Kolcun, R. - Lukáš, D. - Nečas, A. - Amler, Evžen
Needleless emulsion electrospinning for the regulated delivery of susceptible proteins.
Journal of Tissue Engineering and Regenerative Medicine. Roč. 12, č. 3 (2018), s. 583-597. ISSN 1932-6254. E-ISSN 1932-7005
Grant CEP: GA ČR(CZ) GA15-15697S; GA MŠMT(CZ) LO1508; GA MŠMT(CZ) LO1309
Institucionální podpora: RVO:68378041
Klíčová slova: emulsion electrospinning * growth factor * needleless core * shell electrospinning
Obor OECD: Civil engineering
Impakt faktor: 3.319, rok: 2018

In the present work, we developed a novel needleless emulsion electrospinning technique that improves the production rate of the core/shell production process. The nanofibres are based on poly-epsilon-caprolactone PCL as a continuous phase combined with a droplet phase based on Pluronic F-68 PF-68. The PCL-PF-68 nanofibres show a time-regulated release of active molecules. Needleless emulsion electrospinning was used to encapsulate a diverse set of compounds to the core phase i.e. 5- 4,6-dichlorotriazinyl aminofluorescein -PF-68, horseradish peroxidase, Tetramethylrhodamine-dextran, insulin growth factor-I, transforming growth factor- and basic fibroblast growth factor. In addition, the PF-68 facilitates the preservation of the bioactivity of delivered proteins. The system's potential was highlighted by an improvement in the metabolic activity and proliferation of mesenchymal stem cells. The developed system has the potential to deliver susceptible molecules in tissue-engineering applications.
Trvalý link: http://hdl.handle.net/11104/0285454