Proteomic Profiling and Analytical Chemistry: The Crossroads

0477130 - MBÚ 2017 RIV NL eng M - Část monografie knihy
Suder, P. - Novák, Petr - Havlíček, Vladimír - Bodzoń-Kułakowska, A.
General Strategies for Proteomic Sample Preparation.
Proteomic Profiling and Analytical Chemistry: The Crossroads. 2. Amsterdam: Elsevier, 2016, s. 52-62. ISBN 978-044463688-1
Grant CEP: GA MŠMT(CZ) LO1509
Klíčová slova: chromatography * extraction * sample type * proteomics
Kód oboru RIV: EE - Mikrobiologie, virologie

Following experimental design, sample preparation for proteomic analyses is first critical step determining the overall quality of the proteomic output. A broad array of methods ranging from crude protein extraction through separations and sample clean-up techniques to sample enrichment have been discussed in this chapter. Brief assessment of pros and cons of these methods are provided. This chapter also guides the readers through the entire procedure from sample choice to analysis to method optimization.

Trvalý link: http://hdl.handle.net/11104/0273510