Highly Efficient Neural Conversion of Human Pluripotent Stem Cells in Adherent and Animal-Free Conditions

0477003 - ÚEM 2018 RIV US eng J - Článek v odborném periodiku
Lukovic, D. - Diez Lloret, A. - Stojkovic, P. - Rodríguez-Martínez, D. - Arago, M.P.P. - Rodriguez-Jiménez, F.J. - González-Rodríguez, P. - López-Barneo, J. - Syková, Eva - Jendelová, Pavla - Kostic, J. - Moreno-Manzano, V. - Stojkovic, M. - Bhattacharya, S.S. - Erceg, Slaven
Highly Efficient Neural Conversion of Human Pluripotent Stem Cells in Adherent and Animal-Free Conditions.
Stem Cells Translational Medicine. Roč. 6, č. 4 (2017), s. 1217-1226. ISSN 2157-6564. E-ISSN 2157-6580
Grant CEP: GA ČR(CZ) GBP304/12/G069; GA MŠMT(CZ) LM2015064; GA MŠMT(CZ) ED1.1.00/02.0109
Institucionální podpora: RVO:68378041
Klíčová slova: cellular therapy * clinical translation * differentiation
Obor OECD: Developmental biology
Impakt faktor: 4.929, rok: 2017

Neural differentiation of human embryonic stem cells (hESCs) and induced pluripotent stem cells (hiPSCs) can produce a valuable and robust source of human neural cell subtypes, holding great promise for the study of neurogenesis and development, and for treating neurological diseases. However, current hESCs and hiPSCs neural differentiation protocols require either animal factors or embryoid body formation, which decreases efficiency and yield, and strongly limits medical applications. Here we develop a simple, animal-free protocol for neural conversion of both hESCs and hiPSCs in adherent culture conditions. A simple medium formula including insulin induces the direct conversion of >98% of hESCs and hiPSCs into expandable, transplantable, and functional neural progenitors with neural rosette characteristics. Further differentiation of neural progenitors into dopaminergic and spinal motoneurons as well as astrocytes and oligodendrocytes indicates that these neural progenitors retain responsiveness to instructive cues revealing the robust applicability of the protocol in the treatment of different neurodegenerative diseases. The fact that this protocol includes animal-free medium and human extracellular matrix components avoiding embryoid bodies makes this protocol suitable for the use in clinic.
Trvalý link: http://hdl.handle.net/11104/0273402