Interaction of human osteoblast-like Saos-2 cells with stainless steel coated by silicalite-1 films

0476869 - ÚFCH JH 2018 RIV CH eng J - Článek v odborném periodiku
Jirka, Ivan - Vandrovcová, Marta - Plšek, Jan - Bouša, Milan - Brabec, Libor - Dragounová, Helena - Bačáková, Lucie
Interaction of human osteoblast-like Saos-2 cells with stainless steel coated by silicalite-1 films.
Materials Science & Engineering C-Materials for Biological Applications. Roč. 76, JUL 2017 (2017), s. 775-781. ISSN 0928-4931. E-ISSN 1873-0191
Grant CEP: GA ČR(CZ) GA16-02681S
Institucionální podpora: RVO:61388955 ; RVO:67985823
Klíčová slova: silicalite-1 film * biocompatibility * human osteoblast-like Saos-2 cells
Obor OECD: Physical chemistry; Biomaterials (as related to medical implants, devices, sensors) (FGU-C)
Impakt faktor: 5.080, rok: 2017

This paper investigates the interaction of human osteoblast-like Saos-2 cells with stainless steel covered by a film of densely inter-grown silicalite-1 crystals with defined outer and inner surfaces. The chemical composition of this film, labeled as SF(RT), was tuned by heat treatment at 300 °C and 500 °C (labeled as SF(300) and SF(500), respectively) and characterized by X-ray photoelectron spectroscopy (XPS), water drop contact angle (WCA) measurements and scanning electron microscopy (SEM). The number, the spreading area and the activity of alkaline phosphatase of human osteoblast-like Saos-2 cells in cultures on the silicalite-1 film were affected by the chemical composition of its outer surface and by its micro-porous structure. The number and the spreading area of the adhered osteoblast-like cells on day 1 was highest on the surface of SF(RT) relative to their adhesion and spreading on a glass cover slip due to the SF(RT) topology. However, SF(300) markedly supported cell growth during days 3 and 7 after seeding.
Trvalý link: http://hdl.handle.net/11104/0273284