Immobilization of Irpex lacteus to liquid-core alginate beads and their application to degradation of pollutants

0476206 - MBÚ 2018 RIV CZ eng J - Článek v odborném periodiku
Šíma, J. - Milne, R. - Novotný, Čeněk - Hasal, P.
Immobilization of Irpex lacteus to liquid-core alginate beads and their application to degradation of pollutants.
Folia Microbiologica. Roč. 62, č. 4 (2017), s. 335-342. ISSN 0015-5632. E-ISSN 1874-9356
Institucionální podpora: RVO:61388971
Klíčová slova: WHITE-ROT FUNGI * ROTATING BIOLOGICAL CONTACTOR * PHANEROCHAETE-CHRYSOSPORIUM
Obor OECD: Microbiology
Impakt faktor: 1.311, rok: 2017

White rot fungi (WRF) are applicable to biodegradation of recalcitrant pollutants. However, excessive biomass growth typical for WRF cultivation can hinder their large scale applications. Therefore, immobilization of Irpex lacteus to liquid-core alginate beads restricting excessive mycelium growth and simultaneously keeping high degradation rate of pollutants was tested. Effective diffusivities of dyes to the beads varied from (2.98 +/- 0.69) x 10(-10) to (10.27 +/- 2.60) x 10(-10) m(2)/s. Remazol Brilliant Blue R (RBBR), Reactive Orange 16 (RO16), and Naphthol Blue Black (NBB) were used as model dyes. The immobilized fungus decolorized model dyes when applied both in microwell plates and in fluidized bed reactors. Using the microwell plates, the apparent reaction rate constants ranged from (2.06 +/- 0.11) x 10(-2) to (11.06 +/- 0.27) x 10(-2) 1/h, depending on the dye used and its initial concentration. High initial concentrations negatively affected the dye decolorization rate. No fungal growth outside the beads was observed in fluidized bed reactors and thus no operational problems linked to an excessive biomass growth occurred. When RBBR was decolorized in subsequent batches in the fluidized bed reactor, the apparent reaction rate constant increased from (11.63 +/- 0.35) x 10(-2) to (29.26 +/- 7.19) x 10(-2) 1/h.
Trvalý link: http://hdl.handle.net/11104/0272732