Počet záznamů: 1  

Unexpected Position-Dependent Effects of Ribose G-Quartets in G-Quadruplexes

  1. 1.
    0476154 - BFÚ 2018 US eng J - Článek v odborném periodiku
    Zhou, J. - Amrane, S. - Rosu, F. - Salgado, G. - Bian, Y. - Tateishi-Karimata, H. - Largy, E. - Korkut, D. N. - Bourdoncle, A. - Miyoshi, D. - Zhang, J. - Ju, H. - Wang, W. - Sugimoto, N. - Gabelica, V. - Mergny, Jean-Louis
    Unexpected Position-Dependent Effects of Ribose G-Quartets in G-Quadruplexes.
    Journal of the American Chemical Society. Roč. 139, č. 23 (2017), s. 7768-7779. ISSN 0002-7863. E-ISSN 1520-5126
    Institucionální podpora: RVO:68081707
    Klíčová slova: human telomeric rna * electrospray mass-spectrometry * molecular crowding conditions * dna g-quadruplexes
    Obor OECD: Electrochemistry (dry cells, batteries, fuel cells, corrosion metals, electrolysis)
    Impakt faktor: 14.357, rok: 2017

    To understand the role of ribose G-quartets and how they affect the properties of G-quadruplex, structures, we studied three systems in which one, two, three, or four deoxyribose G-quartets were substituted-with ribose G-quartets. These systems were a parallel DNA intramolecular G-quadruplex, d(TTGGG-TGGGTTGGGTGGGTT), and two tetramolecular G-quadruplexes, d(TGGGT) and cl(TQGGGT). Thermal denaturation experiments revealed that ribose G-quartets have position dependent and cumulative effects on G-quadruplex stability. An unexpected destabilization was observed when rG quartets were presented at the 5'-end of the G stack. This observation challenges the general belief that RNA residues, stabilize G-quadruplexes. Furthermore, in contrast to past proposals, hydration is not the main factor determining the stability of our RNA/DNA chimeric G-quadruplexes. Interestingly, the presence of rG residues in a central G-quartet facilitated the formation of additional tetramolecular G-quadruplex topologies showing positive circular dichroism signals at 295 nm. 2D NMR analysis of the tetramolecular TGgGGT (lowercase letter indicates ribose) indicates that Gs in the 5'-most G-quartet adopt the syn conformation. These analyses highlight several new aspects of the role of ribose G-quartets on G-quadruplex structure and stability, and demonstrate that the positions of ribose residues are critical for tuning G-quadruplex properties.
    Trvalý link: http://hdl.handle.net/11104/0272687

     
     
Počet záznamů: 1  

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