The extent of the temperature-induced membrane remodeling in two closely related Bordetella species reflects their adaptation to diverse environmental niches

0476018 - MBÚ 2018 RIV US eng J - Článek v odborném periodiku
Seydlová, G. - Beranová, I. - Bíbová, Ilona - Dienstbier, Ana - Držmíšek, Jakub - Mašín, Jiří - Fišer, R. - Konopásek, I. - Večerek, Branislav
The extent of the temperature-induced membrane remodeling in two closely related Bordetella species reflects their adaptation to diverse environmental niches.
Journal of Biological Chemistry. Roč. 292, č. 19 (2017), s. 8048-8058. ISSN 0021-9258. E-ISSN 1083-351X
Grant CEP: GA ČR(CZ) GF16-34825L; GA ČR(CZ) GA16-05919S; GA MZd(CZ) NV16-30782A; GA MŠMT(CZ) EE2.3.20.0055; GA MŠMT(CZ) EE2.3.30.0003; GA MŠMT(CZ) ED1.1.00/02.0109
Institucionální podpora: RVO:61388971
Klíčová slova: PERTUSSIS ADENYLATE-CYCLASE * ESCHERICHIA-COLI * FATTY-ACIDS
Obor OECD: Microbiology
Impakt faktor: 4.011, rok: 2017

Changes in environmental temperature represent one of the major stresses faced by microorganisms as they affect the function of the cytoplasmic membrane. In this study, we have analyzed the thermal adaptation in two closely related respiratory pathogens Bordetella pertussis and Bordetella bronchiseptica. Although B. pertussis represents a pathogen strictly adapted to the human body temperature, B. bronchiseptica causes infection in a broad range of animals and survives also outside of the host. We applied GC-MS to determine the fatty acids of both Bordetella species grown at different temperatures and analyzed the membrane fluidity by fluorescence anisotropy measurement. In parallel, we also monitored the effect of growth temperature changes on the expression and production of several virulence factors. In response to low temperatures, B. pertussis adapted its fatty acid composition and membrane fluidity to a considerably lesser extent when compared with B. bronchiseptica. Remarkably, B. pertussis maintained the production of virulence factors at 24 degrees C, whereas B. bronchiseptica cells resumed the production only upon temperature upshift to 37 degrees C. This growth temperature-associated differential modulation of virulence factor production was linked to the phosphorylation state of transcriptional regulator BvgA. The observed differences in low-temperature adaptation between B. pertussis and B. bronchiseptica may result from selective adaptation of B. pertussis to the human host. We propose that the reduced plasticity of the B. pertussis membranes ensures sustained production of virulence factors at suboptimal temperatures and may play an important role in the transmission of the disease.
Trvalý link: http://hdl.handle.net/11104/0272597