Prostaglandin E2 stimulates the expression of cumulus expansion-related genes in pigs: the role of protein kinase B

0475936 - ÚŽFG 2018 RIV US eng J - Článek v odborném periodiku
Blaha, Milan - Procházka, Radek - Adámková, K. - Nevoral, J. - Němcová, Lucie
Prostaglandin E2 stimulates the expression of cumulus expansion-related genes in pigs: the role of protein kinase B.
Prostaglandins & Other Lipid Mediators. Roč. 130, č. 2 (2017), s. 38-46. ISSN 1098-8823. E-ISSN 2212-196X
Grant CEP: GA MZe(CZ) QJ1510138; GA MŠMT EF15_003/0000460
Institucionální podpora: RVO:67985904
Klíčová slova: cumulus * oocyte * prostaglandin E2 * protein kinase B
Obor OECD: Reproductive biology (medical aspects to be 3)
Impakt faktor: 2.991, rok: 2017

The production of prostaglandin E2 (PGE2) seems to play an important role in the ovulation process. PGE2 was found to induce cumulus expansion and meiosis resumption in mice, but little is known about its role in pigs. The goals of this study were (a) to assess the effect of PGE2 on the expression levels of cumulus expansion-related genes, (b) to define the signaling pathways that drive the PGE2-stimulated expression of cumulus expansion related genes, (c) to measure the effect of PGE2 on the activation of key signaling molecules (MAPK3/1, PKB) and on hyaluronan production in cumulus cells, and (d) to assess the effect of PGE2 on meiosis resumption. We documented that PGE2 is able to induce the expression of cumulus expansion-related genes (HAS2, TNFAIP6) as well as genes involved in steroidogenesis (CYP11A1) or prostaglandin production (PTGS2). PGE2 is able to activate PKB and MAPK3/1 and induce mild cumulus expansion and meiosis resumption, but less efficiently than FSH.
Trvalý link: http://hdl.handle.net/11104/0272525