Glycosidases in porcine follicular fluid and their effect on zona pellucida-AWN 1 spermadhesin interaction.

0475858 - BTÚ 2018 RIV NL eng J - Článek v odborném periodiku
Dráb, T. - Ren, Štěpán - Maňásková-Postlerová, Pavla - Tichá, M. - Jonáková, Věra - Liberda, J.
Glycosidases in porcine follicular fluid and their effect on zona pellucida-AWN 1 spermadhesin interaction.
Theriogenelogy. Roč. 100, Sep 15 2017 (2017), s. 80-87. ISSN 0093-691X. E-ISSN 1879-3231
Grant CEP: GA ČR GA14-05547S; GA MŠMT(CZ) ED1.1.00/02.0109
Institucionální podpora: RVO:86652036
Klíčová slova: glycosidases * follicle * oocyte development * zona pellucida * AWN 1 spermadhesin
Obor OECD: Biochemical research methods
Impakt faktor: 2.136, rok: 2017

Oligosaccharide moieties on the surface of the oocyte belong to the key molecules that direct the course of fertilization and are subjected to changes during oocyte maturation in the follicle. In our study, we focused on the activities of five glycosidases in the fluids from porcine secondary and preovulatory follicles (a-L-fucosidase, a-D-galactosidase, b-D-galactosidase, b-D-N- ylhexosaminidase, and a-Dmannosidase). All of them were detected active at neutral and acidic pH. However, changes in their activities associated with follicle development were observed only in the case of a-D-mannosidase, which was increased (P < 0.001), and b-D-galactosidase, which was decreased (P < 0.001) at neutral pH, and of a-D-galactosidase and b-N-acetylhexosaminidase, which were decreased (P < 0.0001) at the acidic pH. The comparison of glycosidases from follicular fluid and from blood plasma using red native electrophoresis revealed that most of the glycosidases are present in more than one isoenzyme form, some of them were detected mainly in the follicular fluid. Finally, we tested the effect of glycosidases on the interaction between zona pellucida and AWN 1 spermadhesin (putative sperm receptor of zona pellucida) and demonstrated that the effect of both b-D-galactosidase and to a lesser degree a-D-mannosidase led to a decrease in this interaction. We can hypothesize that these two glycosidases modulate the amount of zona pellucida oligosaccharide moieties and/or their structures for an optimal sperm binding in pigs.
Trvalý link: http://hdl.handle.net/11104/0273880