Differential effects of carbohydrates on arabidopsis pollen germination

0475306 - ÚVGZ 2018 RIV JP eng J - Článek v odborném periodiku
Hirsche, J. - Fernández, J. M. G. - Stabentheiner, E. - Großkinsky, D.K. - Roitsch, Thomas
Differential effects of carbohydrates on arabidopsis pollen germination.
Plant and Cell Physiology. Roč. 58, č. 4 (2017), s. 691-701. ISSN 0032-0781. E-ISSN 1471-9053
Grant CEP: GA MŠMT(CZ) LO1415
Institucionální podpora: RVO:67179843
Klíčová slova: Arabidopsis thaliana * Carbohydrates * Metabolic regulation * Pollen germination * Signaling * Structure-function relationship
Obor OECD: Cell biology
Impakt faktor: 4.059, rok: 2017

Pollen germination as a crucial process in plant development strongly depends on the accessibility of carbon as energy source. Carbohydrates, however, function not only as a primary energy source, but also as important signaling components. In a comprehensive study, we analyzed various aspects of the impact of 32 different sugars on in vitro germination of Arabidopsis pollen comprising about 150 variations of individual sugars and combinations. Twenty-six structurally different mono-, di- and oligosaccharides, and sugar analogs were initially tested for their ability to support pollen germination. Whereas several di- and oligosaccharides supported pollen germination, hexoses such as glucose, fructose and mannose did not support and even considerably inhibited pollen germination when added to germination-supporting medium. Complementary experiments using glucose analogs with varying functional features, the hexokinase inhibitor mannoheptulose and the glucose-insensitive hexokinase-deficient Arabidopsis mutant gin2-1 suggested that mannose- and glucose-mediated inhibition of sucrose-supported pollen germination depends partially on hexokinase signaling. The results suggest that, in addition to their role as energy source, sugars act as signaling molecules differentially regulating the complex process of pollen germination depending on their structural properties. Thus, a sugar-dependent multilayer regulation of Arabidopsis pollen germination is supported, which makes this approach a valuable experimental system for future studies addressing sugar sensing and signaling. © The Author 2017. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists.
Trvalý link: http://hdl.handle.net/11104/0272143