Počet záznamů: 1  

Tuning the hybridization properties of modified oligonucleotides: from flexible to conformationally constrained phosphonate internucleotide linkages

  1. 1.
    0475163 - ÚOCHB 2018 RIV GB eng J - Článek v odborném periodiku
    Páv, Ondřej - Barvík, I. - Liboska, Radek - Petrová, Magdalena - Šimák, Ondřej - Rosenbergová, Šárka - Novák, Pavel - Buděšínský, Miloš - Rosenberg, Ivan
    Tuning the hybridization properties of modified oligonucleotides: from flexible to conformationally constrained phosphonate internucleotide linkages.
    Organic & Biomolecular Chemistry. Roč. 15, č. 3 (2017), s. 701-707. ISSN 1477-0520. E-ISSN 1477-0539
    Grant CEP: GA ČR GA13-26526S; GA MZd NV15-31604A
    Institucionální podpora: RVO:61388963
    Klíčová slova: molecular dynamics simulations * sugar phosphate backbone * nucleic acid recognition
    Obor OECD: Organic chemistry
    Impakt faktor: 3.423, rok: 2017

    The concept of conformational restriction leading to the preorganization of modified strands has proven to be successful and has afforded nucleic acid analogues with many interesting properties suitable for various biochemical applications. We utilized this concept to prepare a set of constrained oligonucleotides derived from 1,4-dioxane and 1,3-dioxolane-locked nucleoside phosphonates and evaluated their hybridization affinities towards their complementary RNA strands. With an increase of Delta T-m per modification up to + 5.2 degrees C, the hybridization experiments revealed the (S)-2', 3'-O-phosphonomethylidene internucleotide linkage as one of the most T-m-increasing modifications reported to date. Moreover, we introduced a novel prediction tool for the pre-selection of potentially interesting chemical modifications of oligonucleotides.
    Trvalý link: http://hdl.handle.net/11104/0272011

     
     
Počet záznamů: 1  

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