Localization of ezrin/radixin/moesin in sperm and their possible association with molecules involved in sperm-egg interaction.

0474846 - BTÚ 2018 CZ eng A - Abstrakt
Šimoník, Ondřej - Dorosh, Andriy - Frolíková, Michaela - Děd, Lukáš - Dvořáková-Hortová, Kateřina
Localization of ezrin/radixin/moesin in sperm and their possible association with molecules involved in sperm-egg interaction.
Book of abstracts XXIIIrd Symposium of Immunology and Biology of Reproduction. Vestec u Prahy: Biotechnologický ústav AVČR v. v. i., 2017 - (Kubátová, a.). s. 28-29
[XXIIIrd Symposium of Immunology and Biology of Reproduction. 18.05.2017-20.05.2017, Třešť]
Institucionální podpora: RVO:86652036
Klíčová slova: monoclonal antibodies * sperm-zona pellucida receptors * ZP glycoproteins * spermatozoa * capacitation * sperm surface proteins
Obor OECD: Biochemistry and molecular biology

Although there has been recently made a great progress in knowledge of mammalian fertilization, the mammalian gametes’ interaction during their fusion has not been fully comprehended yet. The aim of our study is to localize Ezrin, Radixin and Moesin (ERM) proteins in sperm of different species and to perform a co-immunoprecipitation assay to determine a predicted interaction with proteins involved in sperm-egg interaction. Prior to fertilization, sperm have to undergo capacitation and acrosome reaction, among which actin polymerization and depolymerization plays an important role. ERM protein family acts as cross-linkers of actin and the plasma membrane. Our results of immuno-localization and Western blot demonstrate a presence of ezrin and other members of ERM family in mouse, boar and bull sperm. First selected molecule for co-immunoprecipitation assay was CD46 which is suspected to interact with ERM during sperm-egg fusion due to its described role in acrosome integrity transduction pathways and processes before the fusion of gametes. Other proteins, which are likely to interact with ERM, belong to the tetraspanin family. These proteins are essential for successful fertilization as major coordinators of a molecular network which consists of receptors or fusogens. Specifically, CD9 and CD81 are the key tetraspanins during the fusion process. CD81 is complementary to CD9 and their interaction with ERM throughout a family of four cell-surface immunoglobulin superfamily proteins, sharing a conserved glutamine-tryptophan-isoleucine motif and lining them with actin core is described in leukocytes. Based on our ERM immune-localization in sperm, we hypothesise that these proteins could interact with aforementioned tetraspanins and play important part during sperm-egg interaction and fusion. We further aim to characterise the interaction between ERM and fusion relevant molecules which could help to reveal unknown reasons connected to idiopathic infertility issues.







Trvalý link: http://hdl.handle.net/11104/0276723