The alterations in protamination and expression of selected testicular genes in diabetic mice in multigenerational study.

0474839 - BTÚ 2018 CZ eng A - Abstrakt
Valášková, Eliška - Žatecká, Eva - Dorosh, Andriy - Pavlínková, Gabriela - Bohuslavová, Romana - Elzeinová, Fatima - Margaryan, Hasmik - Dvořáková-Hortová, Kateřina - Pěknicová, Jana
The alterations in protamination and expression of selected testicular genes in diabetic mice in multigenerational study.
Book of abstracts XXIIIrd Symposium of Immunology and Biology of Reproduction. Vestec u Prahy: Biotechnologický ústav AVČR v. v. i., 2017 - (Kubátová, a.). s. 19-20
[XXIIIrd Symposium of Immunology and Biology of Reproduction. 18.05.2017-20.05.2017, Třešť]
Grant CEP: GA MŠMT(CZ) ED1.1.00/02.0109; GA MZd(CZ) AZV 15-30880A
Institucionální podpora: RVO:86652036
Klíčová slova: diabetes mellitus * protamination * gene expression * spermatogenesis * spermiogenesis
Obor OECD: Biochemistry and molecular biology

According to the World Health Organization (WHO), 15% of couples in reproductive age suffer from infertility problems, and up to 60% cases are due to male infertility. Causes of this condition could be genetic background, environmental factors and various diseases, including diabetes mellitus (DM). The aim of this multigenerational study was to investigate the effects of DM on sperm parameters and expression of selected testicular genes using streptozotocin-induced type 1 diabetes mouse model and reveal possible paternal transmission to subsequent male generations.
The critical and unique step during spermatogenesis, especially in the elongation of spermatids, is a replacement of the most histones by protamines (PRMs). This process involves transition proteins (TNPs), which help to bind protamines to form a highly compact nucleoprotamine complex. On mRNA level and on protein level as well, we assessed the qualitative and quantitative changes of these essential sperm nuclear proteins, which condense chromatin and may play role in epigenetic regulations. Protamine 1 to protamine 2 ratio (P1/P2), a marker of male fertility, was changed in the sperm not only in the parental diabetic generation, but also in subsequent F1 and F2 offspring generations. Moreover, based on qPCR, in diabetic animals, we found alterations in the gene expression of Prm1 and Prm2 compared to controls in parental generation.
Our results have shown that diabetic condition caused changes in protamination in sperm, and in the gene expression of Prm1 and Prm2 as well. We have also observed alterations in genes important during a meiotic phase of spermatogenesis (Sycp1 and Sycp3) and genes important during spermiogenesis (Tnp1, Tnp2). We have observed transgenerational effect through the protamine ratio and changes in the gene expression of Prm1. These transmitted alterations indicate increased susceptibility to the infertility to next generation via male germ cells.






Trvalý link: http://hdl.handle.net/11104/0276731