Carcinoma cellular uptake, imaging, and targeting by RGD- and TAT-conjugated upconversion and/or magnetic nanoparticles

0474727 - ÚMCH 2018 RIV US eng C - Konferenční příspěvek (zahraniční konf.)
Horák, Daniel - Kostiv, Uliana - Proks, Vladimír - Jirák, D.
Carcinoma cellular uptake, imaging, and targeting by RGD- and TAT-conjugated upconversion and/or magnetic nanoparticles.
TechConnect Briefs 2017. Danville: TechConnect, 2017, s. 114-117. ISBN 978-0-9988782-0-1.
[TechConnect World Innovation Conference & Expo. Nanotech 2017. Washington (US), 14.05.2017-17.05.2017]
Grant CEP: GA ČR(CZ) GA15-01897S
Institucionální podpora: RVO:61389013
Klíčová slova: upconversion * magnetic * nanoparticles
Obor OECD: Polymer science
https://briefs.techconnect.org/papers/carcinoma-cellular-uptake-imaging-and-targeting-by-rgds-and-tat-conjugated-upconversion-and-or-magnetic-nanoparticles/

We have developed monodisperse magnetic Fe3O4, upconversion NaYF4:Yb3+/Er3+, and dual paramagnetic/upconversion NaGdF4: Yb3+/Er3+ nanoparticles with tunable size by thermal decomposition of Fe(III) oleate and lanthanide chlorides in high-boiling organic solvents. Aminosilica and 4-pentynoic acid were then introduced on the particle surface to conjugate the cell adhesive and cell penetrating RGD and TAT peptides, respectively. Optionally, NaYF4:Yb3+/Er3+@SiO2-NH2 particles reacted with methoxyPEG succinimidyl ester to diminish uptake of the particles by the reticulo-endothelial system. Neural stem cell viability and labeling efficiency of the particles were determined. Al carboxyphthalocyanine was conjugated to NaYF4:Yb37Er3+@SiO2-PEG nanoparticles, which were intravenously administered in athymic nude mice with xenotransplanted human prostate carcinoma PC-3. After NIR photodynamic therapy, the tumor subsided and its volume approached to zero.
Trvalý link: http://hdl.handle.net/11104/0280742