Počet záznamů: 1  

Trilobolide-steroid hybrids: Synthesis, cytotoxic and antimycobacterial activity

  1. 1.
    0473082 - ÚMG 2017 RIV US eng J - Článek v odborném periodiku
    Jurášek, M. - Džubák, P. - Rimpelová, S. - Sedlák, David - Konečný, P. - Frydrych, I. - Gurska, S. - Hajdúch, M. - Bogdanová, K. - Kolář, M. - Muller, Tomáš - Kmoníčková, Eva - Ruml, T. - Harmatha, Juraj - Drašar, P. B.
    Trilobolide-steroid hybrids: Synthesis, cytotoxic and antimycobacterial activity.
    Steroids. Roč. 117, JAN (2017), s. 97-104. ISSN 0039-128X. E-ISSN 1878-5867
    Grant CEP: GA MŠMT(CZ) LO1304; GA MŠMT LO1220; GA MŠMT LM2015063
    Grant ostatní: GA ČR(CZ) GA14-04329S
    Institucionální podpora: RVO:68378050 ; RVO:68378041 ; RVO:61388963
    Klíčová slova: Trilobolide * Steroids * Click chemistry * Cytotoxicity * sar * Steroid receptor
    Obor OECD: Biochemistry and molecular biology; Organic chemistry (UOCHB-X)
    Impakt faktor: 2.523, rok: 2017

    Sesquiterpene lactone trilobolide is a sarco/endoplasmic reticulum Ca2+-ATPase (SERCA) inhibitor, thus depleting the Ins(1,4,5)P3-sensitive intracellular calcium stores. Here, we describe a synthesis of a series of 6 trilobolide-steroids conjugates (estradiol, pregnene, dehydroepiandrosterone, and testosterone). We found that the newly synthesized Tb-based compounds possess different remarkable biologital activities. Cancer cell cytotoxicity and preferential selectivity is represented in our study by a Tb-pregnene derivative. The most cytotoxic clickates of estradiol and pregnene were studied by FACS where impact on cell cycle and RNA synthesis was observed; live-cell microscopy revealed the impact on cell organelle morphology particularly endoplasmic reticulum, mitochondria and nucleus. Further, we have studied the estrogenic and androgenic properties of the cliCkate molecules using cell-based luciferase assays. Finally, antimycobacterial tests revealed that testosterone and estradiol derivatives potentiated the antimycobacterial activity up to IC50 of 10.6 mu M. (C) 2016 Elsevier Inc. All rights reserved.
    Trvalý link: http://hdl.handle.net/11104/0270248

     
     
Počet záznamů: 1  

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