Poly(amino acid)-based fibrous scaffolds modified with surface-pendant peptides for cartilage tissue engineering

0472812 - ÚMCH 2018 RIV GB eng J - Článek v odborném periodiku
Svobodová, Jana - Proks, Vladimír - Karabiyik, Ö. - Calikoglu Koyuncu, A. C. - Köse, G. T. - Rypáček, František - Studenovská, Hana
Poly(amino acid)-based fibrous scaffolds modified with surface-pendant peptides for cartilage tissue engineering.
Journal of Tissue Engineering and Regenerative Medicine. Roč. 11, č. 3 (2017), s. 831-842. ISSN 1932-6254. E-ISSN 1932-7005
Grant CEP: GA ČR GAP108/12/1629; GA ČR GAP108/12/1538
Grant ostatní: AV ČR, TUBITAK(CZ) 111M031
Institucionální podpora: RVO:61389013
Klíčová slova: poly(amino acid) * fibrous scaffolds * adhesion peptide
Obor OECD: Polymer science
Impakt faktor: 4.089, rok: 2017

In this study, fibrous scaffolds based on poly(gamma-benzyl-l-glutamate) (PBLG) were investigated in terms of the chondrogenic differentiation potential of human tooth germ stem cells (HTGSCs). Through the solution-assisted bonding of the fibres, fully connected scaffolds with pore sizes in the range 20–400 µm were prepared. Biomimetic modification of the PBLG scaffolds was achieved by a two-step reaction procedure: first, aminolysis of the PBLG fibres’ surface layers was performed, which resulted in an increase in the hydrophilicity of the fibrous scaffolds after the introduction of N5-hydroxyethyl-l-glutamine units. Second, modification with the short peptide sequence azidopentanoyl–GGGRGDSGGGY–NH2, using the 'click' reaction on the previously modified scaffold with 2-propynyl side-chains, was performed. Radio-assay of the 125I-labelled peptide was used to evaluate the RGD density in the fibrous scaffolds (which varied in the range 10–3–10 pm/cm2). All the PBLG scaffolds, especially with density 90 ± 20 fm/cm2 and 200 ± 100 fm/cm2 RGD, were found to be potentially suitable for growth and chondrogenic differentiation of HTGSCs.
Trvalý link: http://hdl.handle.net/11104/0270733