Conferring resistance to geminiviruses with the CRISPR-Cas prokaryotic immune system

0472038 - BFÚ 2017 RIV GB eng J - Článek v odborném periodiku
Baltes, N.J. - Hummel, A.W. - Konecna, E. - Čegan, Radim - Bruns, A.N. - Bisaro, D.M. - Voytas, D.F.
Conferring resistance to geminiviruses with the CRISPR-Cas prokaryotic immune system.
Nature Plants. Roč. 1, č. 10 (2015), č. článku 15145. ISSN 2055-026X. E-ISSN 2055-0278
Institucionální podpora: RVO:68081707
Klíčová slova: sequence data * plant * dna
Kód oboru RIV: BO - Biofyzika

To reduce crop losses due to geminivirus infection, we targeted the bean yellow dwarf virus (BeYDV) genome for destruction with the CRISPR-Cas (clustered, regularly interspaced short palindromic repeats-CRISPR-associated proteins) system. Transient assays using BeYDV-based replicons revealed that CRISPR-Cas reagents introduced mutations within the viral genome and reduced virus copy number. Transgenic plants expressing CRISPR-Cas reagents and challenged with BeYDV had reduced virus load and symptoms, thereby demonstrating a novel strategy for engineering resistance to geminiviruses.
Trvalý link: http://hdl.handle.net/11104/0269383