Recent progress in electrochemical sensors and assays for DNA damage and repair

0471986 - BFÚ 2017 RIV GB eng J - Článek v odborném periodiku
Fojta, Miroslav - Daňhel, Aleš - Havran, Luděk - Vyskočil, V.
Recent progress in electrochemical sensors and assays for DNA damage and repair.
TrAC-Trends in Analytical Chemistry. Roč. 79, MAY2016 (2016), s. 160-167. ISSN 0165-9936. E-ISSN 1879-3142
Grant CEP: GA ČR GBP206/12/G151
Institucionální podpora: RVO:68081707
Klíčová slova: in-situ evaluation * carbon-paste electrode * voltammetric detection
Kód oboru RIV: BO - Biofyzika
Impakt faktor: 8.442, rok: 2016

DNA in cells is permanently exposed to endogenous or exogenous chemical or physical agents which may cause chemical changes in the DNA structure collectively called "DNA damage", which may have severe impacts on human health. Therefore, efficient techniques to detect DNA damage are sought, among which electroanalytical methods hold an important position. This review is focused on recent progress in the field of electrochemical sensors and assays for DNA damage, particularly on typical applications of advanced electrochemical techniques in detecting oxidative DNA damage, abasic lesions, base mismatches, non-covalent interactions with drugs and other substances. Special attention is paid to combinations of electrochemical detection with biochemical tools such as enzymatic systems mimicking metabolic activation of xenobiotics, or DNA repair enzymes converting specific nucleobase lesions to species offering more facile electrochemical detection. These applications create conditions for application of electroanalytical approaches in biological research, including studies of DNA repair processes. (C) 2015 Elsevier B.V. All rights reserved.
Trvalý link: http://hdl.handle.net/11104/0269345