Imaging of transfection and intracellular release of intact, non-labeled DNA using fluorescent nanodiamonds

0471415 - FZÚ 2017 RIV GB eng J - Článek v odborném periodiku
Petráková, Vladimíra - Benson, Veronika - Bunček, M. - Fišerová, Anna - Ledvina, Miroslav - Štursa, Jan - Cígler, Petr - Nesládek, M.
Imaging of transfection and intracellular release of intact, non-labeled DNA using fluorescent nanodiamonds.
Nanoscale. Roč. 8, č. 23 (2016), s. 12002-12012. ISSN 2040-3364. E-ISSN 2040-3372
Grant CEP: GA MZd(CZ) NV15-33094A; GA MŠMT LM2015056
Grant ostatní: OP VK(XE) CZ.1.07/2.3.00/20.0306
Institucionální podpora: RVO:68378271 ; RVO:61388971 ; RVO:61389005 ; RVO:61388963
Klíčová slova: single-particle tracking * resonance energy-transfer * sirna delivery * gene delivery * correlation spectroscopy * endosomal escape * mass-production * drug-delivery * quantum dots * living cells
Kód oboru RIV: BM - Fyzika pevných látek a magnetismus; CC - Organická chemie (UOCHB-X); EE - Mikrobiologie, virologie (MBU-M); BG - Jaderná, atomová a mol. fyzika, urychlovače (UJF-V)
Impakt faktor: 7.367, rok: 2016

Efficient delivery of stabilized nucleic acids (NAs) into cells and release of the NA payload are crucial points in the transfection process. Here we report on the fabrication of a nanoscopic cellular delivery carrier that is additionally combined with a label-free intracellular sensor device, based on biocompatible fluorescent nanodiamond particles. The sensing function is engineered into nanodiamonds by using nitrogen-vacancy color centers, providing stable non-blinking luminescence. The device is used for monitoring NA transfection and the payload release in cells. The unpacking of NAs from a poly(ethyleneimine)-terminated nanodiamond surface is monitored using the color shift of nitrogen-vacancy centers in the diamond, which serve as a nanoscopic electric charge sensor.
Trvalý link: http://hdl.handle.net/11104/0268809