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CyanoP is Involved in the Early Steps of Photosystem II Assembly in the Cyanobacterium Synechocystis sp PCC 6803

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    0470774 - MBÚ 2017 RIV JP eng J - Článek v odborném periodiku
    Knoppová, Jana - Yu, J. - Koník, P. - Nixon, P. - Komenda, Josef
    CyanoP is Involved in the Early Steps of Photosystem II Assembly in the Cyanobacterium Synechocystis sp PCC 6803.
    Plant and Cell Physiology. Roč. 57, č. 9 (2016), s. 1921-1931. ISSN 0032-0781. E-ISSN 1471-9053
    Grant CEP: GA ČR GBP501/12/G055; GA MŠMT(CZ) LM2015055; GA MŠMT(CZ) LO1416
    Institucionální podpora: RVO:61388971
    Klíčová slova: Cyanobacteria * CyanoP * Photosynthesis
    Kód oboru RIV: EE - Mikrobiologie, virologie
    Impakt faktor: 4.760, rok: 2016

    Although the PSII complex is highly conserved in cyanobacteria and chloroplasts, the PsbU and PsbV subunits stabilizing the oxygen-evolving Mn4CaO5 cluster in cyanobacteria are absent in chloroplasts and have been replaced by the PsbP and PsbQ subunits. There is, however, a distant cyanobacterial homolog of PsbP, termed CyanoP, of unknown function. Here we show that CyanoP plays a role in the early stages of PSII biogenesis in Synechocystis sp. PCC 6803. CyanoP is present in the PSII reaction center assembly complex (RCII) lacking both the CP47 and CP43 modules and binds to the smaller D2 module. A small amount of larger PSII core complexes co-purifying with FLAG-tagged CyanoP indicates that CyanoP can accompany PSII on most of its assembly pathway. A role in biogenesis is supported by the accumulation of unassembled D1 precursor and impaired formation of RCII in a mutant lacking CyanoP. Interestingly, the pull-down preparations of CyanoP-FLAG from a strain lacking CP47 also contained PsbO, indicating engagement of this protein with PSII at a much earlier stage in assembly than previously assumed.
    Trvalý link: http://hdl.handle.net/11104/0269121

     
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