Influence of commensal Lactobacillus amylovorus, probiotic E.coli Nissle 1917 and Salmonella Typhimurium on transcriptions of claudin-1 and occludin in the intestine of gnotobiotic piglets

0469676 - MBÚ 2017 TH eng A - Abstrakt
Šplíchalová, Alla - Šplíchal, Igor - Slavíková, Věra
Influence of commensal Lactobacillus amylovorus, probiotic E.coli Nissle 1917 and Salmonella Typhimurium on transcriptions of claudin-1 and occludin in the intestine of gnotobiotic piglets.
Microbes for the Benefits of Mankind, Book of abstracts. Phuket: ICOBM, 2016, 2016. s. 139-139. ISBN 1876-2883.
[International Conference on Beneficial Microbes 2016. 31.05.2016-16.06.2016, Phuket]
Grant CEP: GA MŠMT(CZ) LD15090
Institucionální podpora: RVO:61388971
Klíčová slova: Lactobacillus amylovorus * c E.coli Nissle 1917 * Salmonella Typhimurium
Kód oboru RIV: EC - Imunologie

Colostrum-deprived germ-free piglets (GF) were colonized with commensal Lactobacillus amylovorus (LA) or probiotic E. coli Nissle 1917 (EcN) four hours after hysterectomy. Another group of piglets was infected at one week of age with Salmonella Typhimurium (ST) for 24 hours. Bacterial colonization of the intestine of one-week-old gnotobiotic piglets and possible translocation (CFU counting), transcriptions (RT-qPCR) of claudin-1 and occludin, and their protein expression (IF) were evaluated. Surprisingly the transcriptions of claudin-1 and occludin in Salmonella-infected piglets showed quite opposite trend compare to GF piglets
Trvalý link: http://hdl.handle.net/11104/0268286