Tracking biochemical changes correlated with ultra-weak photon emission using metabolomics

0469629 - ÚFE 2017 RIV CH eng J - Článek v odborném periodiku
Burgos, R.C.R. - Červinková, Kateřina - van der Laan, T. - Ramautar, R. - van Wijk, E.P.A. - Cifra, Michal - Koval, S. - Berger, R. - Hankemeier, T. - van der Greef, J.
Tracking biochemical changes correlated with ultra-weak photon emission using metabolomics.
Journal of Photochemistry and Photobiology. B - Biology Section. -, č. 163 (2016), s. 237-245. ISSN 1011-1344. E-ISSN 1873-2682
Grant CEP: GA ČR GA13-29294S
Institucionální podpora: RVO:67985882
Klíčová slova: Ultra-weak photon emission * Capillary electrophoresis-mass spectrometry * HL-60 cells
Kód oboru RIV: BO - Biofyzika
Impakt faktor: 2.673, rok: 2016

Ultra-weak photon emission (UPE) is light emitted spontaneously by biological systems without the use of specific luminescent complexes. UPE is emitted in the near-UV/UV-Vis/near-IR spectra during oxidative metabolic reactions; however, the specific pathways involved in UPE remain poorly understood. Here, we used HL-60 cells, a human promyelocytic cell line that is often used to study respiratory burst, as a model system to measure UPE kinetics together with metabolic changes. HL-60 cells were differentiated into neutrophil-like cells by culturing in all-trans-retinoic acid for 7 days. We then used a targeted metabolomics approach with capillary electrophoresis-mass spectrometry to profile intracellular metabolites in HL-60 cells and to investigate the biochemical changes based on the measured UPE profile. Our analysis revealed that the levels of specific metabolites, including putrescine, creatine, beta-alanine, methionine, hydroxyproline, serine, and S-adenosylmethionine, were significantly altered in HL-60 cells after inducing respiratory burst. A comparison with recorded UPE data revealed that the changes in putrescine, glutathione, sarcosine, creatine, beta-alanine, methionine, and hydroxyproline levels were inversely correlated with the change in UPE intensity. These results suggest that these metabolic pathways, particular the methionine pathway, may play a role in the observed changes in UPE in HL-60 cells and therefore demonstrate the potential for using UPE to monitor metabolic changes
Trvalý link: http://hdl.handle.net/11104/0267453