Highly efficient lipid production in the green alga Parachlorella kessleri: draft genome and transcriptome endorsed by whole-cell 3D ultrastructure

0469260 - MBÚ 2017 RIV GB eng J - Článek v odborném periodiku
Ota, S. - Oshima, K. - Yamazaki, T. - Kim, S. - Yu, Z. - Yoshihara, M. - Takeda, K. - Takeshita, T. - Hirata, A. - Bišová, Kateřina - Zachleder, Vilém - Hattori, M. - Kawano, S.
Highly efficient lipid production in the green alga Parachlorella kessleri: draft genome and transcriptome endorsed by whole-cell 3D ultrastructure.
Biotechnology for Biofuels. Roč. 9, č. 13 (2016), s. 13. E-ISSN 1754-6834
Institucionální podpora: RVO:61388971
Klíčová slova: 3D-TEM * Green alga * Parachlorella kessleri * RNA-seq
Kód oboru RIV: EE - Mikrobiologie, virologie
Impakt faktor: 5.203, rok: 2016

Background: Algae have attracted attention as sustainable producers of lipid-containing biomass for food, animal feed, and for biofuels. Parachlorella kessleri, a unicellular green alga belonging to the class Trebouxiophyceae, achieves very high biomass, lipid, and starch productivity levels. However, further biotechnological exploitation has been hampered by a lack of genomic information. Results: Here, we sequenced the whole genome and transcriptome, and analyzed the behavior of P. kessleri NIES-2152 under lipid production-inducing conditions. The assembly includes 13,057 protein-coding genes in a 62.5-Mbp nuclear genome. Under conditions of sulfur deprivation, lipid accumulation was correlated with the transcriptomic induction of enzymes involved in sulfur metabolism, triacylglycerol (TAG) synthesis, autophagy, and remodeling of light-harvesting complexes. Conclusions: Three-dimensional transmission electron microscopy (3D-TEM) revealed extensive alterations in cellular anatomy accompanying lipid hyperaccumulation. The present 3D-TEM results, together with transcriptomic data support the finding that upregulation of TAG synthesis and autophagy are potential key mediators of the hyperaccumulation of lipids under conditions of nutrient stress.
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Trvalý link: http://hdl.handle.net/11104/0267363