The Assembly Factor SDHAF2 Is Dispensable for Flavination of the Catalytic Subunit of Mitochondrial Complex II in Breast Cancer Cells

0468544 - BTÚ 2017 RIV US eng J - Článek v odborném periodiku
Bezawork-Geleta, A. - Dong, L. - Rohlena, Jakub - Neužil, Jiří
The Assembly Factor SDHAF2 Is Dispensable for Flavination of the Catalytic Subunit of Mitochondrial Complex II in Breast Cancer Cells.
Journal of Biological Chemistry. Roč. 291, č. 41 (2016), s. 21414-21420. ISSN 0021-9258. E-ISSN 1083-351X
Grant CEP: GA ČR GA15-02203S; GA ČR(CZ) GA16-22823S; GA MŠMT(CZ) ED1.1.00/02.0109
Institucionální podpora: RVO:86652036
Klíčová slova: cancer biology * mitochondrial respiratory chain complex * SUCCINATE-UBIQUINONE OXIDOREDUCTASE
Kód oboru RIV: EB - Genetika a molekulární biologie
Impakt faktor: 4.125, rok: 2016

Mitochondrial complex II or succinate dehydrogenase (SDH) is at the crossroads of oxidative phosphorylation and the tricarboxylic acid cycle. It has been shown that Sdh5 (SDHAF2/SDH5 in mammals) is required for flavination of the subunit Sdh1 (SDHA in human cells) in yeast. Here we demonstrate that in human breast cancer cells, SDHAF2/SDH5 is dispensable for SDHA flavination. In contrast to yeast, CRISPR-Cas9 nickase-mediated SDHAF2 KO breast cancer cells feature flavinated SDHA and retain fully assembled and functional complex II, as well as normal mitochondrial respiration. Our data show that SDHA flavination is independent of SDHAF2 in breast cancer cells, employing an alternative mechanism.
Trvalý link: http://hdl.handle.net/11104/0266364