Directed evolution of enzymes using microfluidic chips

0468517 - ÚPT 2017 RIV US eng C - Konferenční příspěvek (zahraniční konf.)
Pilát, Zdeněk - Ježek, Jan - Šmatlo, Filip - Kaňka, Jan - Zemánek, Pavel
Directed evolution of enzymes using microfluidic chips.
20th Slovak-Czech-Polish Optical Conference on Wave and Quantum Aspects of Contemporary Optics (Proceedings of SPIE 10142). Bellingham: SPIE, 2016, s. 1-7, č. článku 101420A. ISSN 0277-786X.
[Slovak-Czech-Polish Optical Conference on Wave and Quantum Aspects of Contemporary Optics /20./. Jasná (SK), 05.09.2016-09.09.2016]
Grant CEP: GA ČR(CZ) GA16-07965S; GA TA ČR TA03010642; GA MŠMT(CZ) LO1212; GA MŠMT ED0017/01/01
Institucionální podpora: RVO:68081731
Klíčová slova: bacteria * dielectrophoresis * engineering * luminiscence * proteins
Kód oboru RIV: BH - Optika, masery a lasery
http://proceedings.spiedigitallibrary.org/proceeding.aspx?articleid=2595346

Enzymes are highly versatile and ubiquitous biological catalysts. They can greatly accelerate large variety of reactions, while ensuring appropriate catalytic activity and high selectivity. These properties make enzymes attractive biocatalysts for a wide range of industrial and biomedical applications. Over the last two decades, directed evolution of enzymes has transformed the field of protein engineering. We have devised microfluidic systems for directed evolution of haloalkane dehalogenases in emulsion droplets. In such a device, individual bacterial cells producing mutated variants of the same enzyme are encapsulated in microdroplets and supplied with a substrate. The conversion of a substrate by the enzyme produced by a single bacterium changes the pH in the droplet which is signalized by pH dependent fluorescence probe. The droplets with the highest enzymatic activity can be separated directly on the chip by dielectrophoresis and the resultant cell lineage can be used for enzyme production or for further rounds of directed evolution. This platform is applicable for fast screening of large libraries in directed evolution experiments requiring mutagenesis at multiple sites of a protein structure.
Trvalý link: http://hdl.handle.net/11104/0266361