Počet záznamů: 1
Antiplasmodial Activity Is an Ancient and Conserved Feature of Tick Defensins
- 1.0468224 - BC 2017 RIV CH eng J - Článek v odborném periodiku
Cabezas Cruz, Alejandro - Tonk, M. - Bouchut, A. - Pierrot, C. - Pierce, R.J. - Kotsyfakis, Michalis - Rahnamaeian, M. - Vilcinskas, A. - Khalife, J. - Valdés, James J.
Antiplasmodial Activity Is an Ancient and Conserved Feature of Tick Defensins.
Frontiers in Microbiology. Roč. 7, 24 October (2016), č. článku 1682. ISSN 1664-302X. E-ISSN 1664-302X
Grant CEP: GA MŠMT(CZ) EE2.3.30.0032
Institucionální podpora: RVO:60077344
Klíčová slova: ticks * defensins * antimicrobial spectrum * ancestral sequence reconstruction * Plasmodium falciparum
Kód oboru RIV: EI - Biotechnologie a bionika
Impakt faktor: 4.076, rok: 2016
Ancestral sequence reconstruction has been widely used to test evolution-based hypotheses. The genome of the European tick vector, Ixodes ricinus, encodes for defensin peptides with diverse antimicrobial activities against distantly related pathogens. These pathogens include fungi, Gram-negative, and Gram-positive bacteria, i.e., a wide antimicrobial spectrum. Ticks do not transmit these pathogens, suggesting that these defensins may act against a wide range of microbes encountered by ticks during blood feeding or off-host periods. As demonstrated here, these I. ricinus defensins are also effective against the apicomplexan parasite Plasmodium falciparum. To study the general evolution of antimicrobial activity in tick defensins, the ancestral amino acid sequence of chelicerate defensins, which existed approximately 444 million years ago, was reconstructed using publicly available scorpion and tick defensin sequences (named Scorpions-Ticks Defensins Ancestor, STiDA). The activity of STiDA was tested against P. falciparum and the same Gram-negative and Gram-positive bacteria that were used for the I. ricinus defensins. While some extant tick defensins exhibit a wide antimicrobial spectrum, the ancestral defensin showed moderate activity against one of the tested microbes, P. falciparum. This study suggests that amino acid variability and defensin family expansion increased the antimicrobial spectrum of ancestral tick defensins.
Trvalý link: http://hdl.handle.net/11104/0266064
Počet záznamů: 1