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Novel, versatile capillary electrophoresis instrument with laser induced fluorescence for analysis of various lipid peroxidation biomarkers
- 1.0467984 - ÚIACH 2017 RIV CZ eng C - Konferenční příspěvek (zahraniční konf.)
Lačná, J. - Foret, František - Kubáň, Petr
Novel, versatile capillary electrophoresis instrument with laser induced fluorescence for analysis of various lipid peroxidation biomarkers.
CECE 2016. 13th International Interdisciplinary Meeting on Bioanalysis. Brno: Ústav analytické chemie AV ČR, v. v. i., 2016 - (Foret, F.; Křenková, J.; Drobníková, I.; Klepárník, K.), s. 163-167. ISBN 978-80-904959-4-4.
[CECE 2016. International Interdisciplinary Meeting on Bioanalysis /13./. Brno (CZ), 17.10.2016-19.10.2016]
Institucionální podpora: RVO:68081715
Klíčová slova: CE-LIF * malondialdehyde * biomarkers * lipids
Kód oboru RIV: CB - Analytická chemie, separace
In this work we have developed a new sensitive method for quantitation of MDA in
biological samples by CE-LIF with LOD about 1.7 nM. First part of the work was
devoted to the construction of a new CE-LIF system. Then the derivatization process
of MDA by TBA was optimized. The ability to use this method for the detection of
MDA in a biological samples, such as EBC and plasma, was also demonstrated. For the
first time it was possible to detect MDA in EBC samples by CE. Other biomarkers are
now being studied.
Trvalý link: http://hdl.handle.net/11104/0265912
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