Full Genome Sequence and sfRNA Interferon Antagonist Activity of Zika Virus from Recife, Brazil

0467649 - BC 2017 RIV US eng J - Článek v odborném periodiku
Donald, C. L. - Brennan, B. - Cumberworth, S. L. - Rezelj, V. V. - Clark, J. J. - Cordeiro, C. - de Oliveira Franca, R. F. - Pena, L. J. - Wilkie, G. S. - Filipe, A. Da S. - Davis, C. - Hughes, J. - Varjak, M. - Selinger, Martin - Zuvanov, L. - Owsianka, A. M. - Patel, A. H. - McLauchlan, J. - Lindenbach, B. D. - Fall, G. - Sall, A. A. - Biek, R. - Rehwinkel, J. - Schnettler, E. - Kohl, A.
Full Genome Sequence and sfRNA Interferon Antagonist Activity of Zika Virus from Recife, Brazil.
PLoS Neglected Tropical Diseases. Roč. 10, č. 10 (2016), č. článku e0005048. ISSN 1935-2735. E-ISSN 1935-2735
Grant CEP: GA ČR GA15-03044S
Institucionální podpora: RVO:60077344
Klíčová slova: regulatory factor-3 * rna interference * flavivirus * infection * dengue * responses * cells * pathogenesis * coinfection * phylogenies
Kód oboru RIV: EE - Mikrobiologie, virologie
Impakt faktor: 3.834, rok: 2016

The outbreak of Zika virus (ZIKV) in the Americas has transformed a previously obscure mosquito-transmitted arbovirus of the Flaviviridae family into a major public health concern. Little is currently known about the evolution and biology of ZIKV and the factors that contribute to the associated pathogenesis. Determining genomic sequences of clinical viral isolates and characterization of elements within these are an important prerequisite to advance our understanding of viral replicative processes and virus-host interactions.
We obtained a ZIKV isolate from a patient who presented with classical ZIKV-associated symptoms, and used high throughput sequencing and other molecular biology approaches to determine its full genome sequence, including non-coding regions. Genome regions were characterized and compared to the sequences of other isolates where available. Furthermore, we identified a subgenomic flavivirus RNA (sfRNA) in ZIKV-infected cells that has antagonist activity against RIG-I induced type I interferon induction, with a lesser effect on MDA-5 mediated action.
The full-length genome sequence including non-coding regions of a South American ZIKV isolate from a patient with classical symptoms will support efforts to develop genetic tools for this virus. Detection of sfRNA that counteracts interferon responses is likely to be important for further understanding of pathogenesis and virus-host interactions.
Trvalý link: http://hdl.handle.net/11104/0265719