Rat animal model myocardial cryonecrosis

0466765 - ÚPT 2017 CZ eng A - Abstrakt
Dvořáková, Lenka - Scheer, P. - Jiřík, Radovan - Dražanová, Eva - Hložková, J. - Starčuk jr., Zenon
Rat animal model myocardial cryonecrosis.
Physiological Research. Fyziologický ústav AV ČR, v. v. i. Roč. 65, č. 4 (2016), 2P. ISSN 0862-8408. E-ISSN 1802-9973.
[Conference about Laboratory Animals /19./. 03.05.2016-05.05.2016, Mušov - Pasohlávky]
Grant CEP: GA MŠMT(CZ) LO1212; GA ČR(CZ) GA16-13830S
Institucionální podpora: RVO:68081731
Klíčová slova: model myocardial cryonecrosis
Kód oboru RIV: BM - Fyzika pevných látek a magnetismus

The coronary artery diseases are the leading cause of the death worldwide. The study of heart failure requires viable animal models that mimic human cardiac diseases, such as myocardial infarction. In our study we are presenting a freeze-thaw injury induced myocardial infarction model – a cryonecrosis model, which was validated by a magnetic resonance examination. Five healthy rats were anesthetized (xylazin 5 mg/ kg BW + ketamin 35 mg/kg BW + diazepam 5 mg/kg BW), intubated using 16 G cannula and were artificially ventilated (TOPO, Kent Scientific). A left-sided thoracotomy (4th ICS) was performed and then after fixation of the pericardium the heart was exposed. Cryonecrosis (CN) was produced by attaching a liquidnitrogen cooled steel tool with the diameter of 6 or 8 mm on the left ventricle (LV) free wall on the area of heart apex for 60 s. Thoracotomy was closed in a standard manner. During postoperative care amoxicilin clavulanate was administrated IM daily and every other day a dose of tolfetamic acid was administrated. Five months after CN, rats were sacrificed and hearts stored in a 10 % solution of formaldehyde. All imaging was performed on a 9.4T NMR system (Bruker-Biospec 94/30 USR). For in vivo measurement IntraGate FLASH was used. Three sets of anatomical images in long-axis, short-axis and four-chamber view
were acquired (FOV 4×4 cm, slice thickness 1 mm, matrix 256×256, TR 40 ms, FA 25°, 10 cardiac phases).
Trvalý link: http://hdl.handle.net/11104/0265007