Using ASL method for monitoring of brain perfusion changes in rat models of schizophrenia

0466764 - ÚPT 2017 CZ eng A - Abstrakt
Dražanová, Eva - Krátká, Lucie - Dvořáková, Lenka - Pistovčáková, J. - Rudá-Kučerová, J. - Micale, V. - Demlová, R. - Kašpárek, T. - Starčuk jr., Zenon
Using ASL method for monitoring of brain perfusion changes in rat models of schizophrenia.
Physiological Research. Fyziologický ústav AV ČR, v. v. i. Roč. 65, č. 4 (2016), 2P. ISSN 0862-8408. E-ISSN 1802-9973.
[Conference about Laboratory Animals /19./. 03.05.2016-05.05.2016, Mušov - Pasohlávky]
Grant CEP: GA MŠMT(CZ) LO1212
Institucionální podpora: RVO:68081731
Klíčová slova: ASL method * schizophrenia
Kód oboru RIV: FH - Neurologie, neurochirurgie, neurovědy

Several animal models simulate schizophrenia pathologies. We used the poly I:C and MAM developmental animal models for our investigation. Using arterial spin labeling (ASL) MRI method we examined brain perfusion changes in both developmental models in rats. The developmental poly I:C model was induced by acute subcutaneous dose of poly I:C (8 mg/kg) to 10 pregnant Wistar rat dams in the 15th day of their pregnancy. Vehicle w as a dministered to 10 controls in the same time period. The MAM model was generated by acute intraperitoneal dose of MAM (22 mg/kg) to 10 pregnant Sprague-Dawley rat dams in the 17th day of their pregnancy. Control animals were obtained in the same way. The adult offspring males were subjected to MRI scanning. MR imaging was performed in an animal 9.4T MR scanner (Bruker Biospin 94/30 USR by Bruker, Ettlingen, Germany) using a receiveonly 2×2 array surface coil (400 ARR R.BR) and a volume transmitter coil. Anatomical images were obtained with a RARE sequence with TR 3500 ms, TE 36.0 ms, image matrix 256×256, slice thickness 1.25 mm, 2D FOV 50.0×20.4 mm, RARE factor 2. ASL was performed with a FAIR-RARE sequence with TR 10000 ms, TE 37.78 ms, image matrix 128×96, slice thickness 1.25 mm, 2D FOV 50.0×20.4 mm; from one axial slice through the brain a set of 15 magnitude images with TI of 30, 50, 100, 200, 300, 500, 700, 900, 1000, 1100, 1500, 1800, 2200, 2800, 3200 ms was used to calculate the tissue blood f low m ap. A ll ASL d ata w ere a nalyzed i n P araVision 5 .1 ( Bruker), A SL b lood f low maps were analyzed in manually drawn brain ROIs by own Matlab R2010a code. MRI data were analyzed in STATISTICA (StatSoft Inc.) software by the Mann-Whitney U nonparametric test (boundary of significance set to p<0.05).
Trvalý link: http://hdl.handle.net/11104/0265005