A versatile high-vacuum cryo transfer system for cryo microscopy and analytics

0465341 - ÚPT 2017 RIV GB eng C - Konferenční příspěvek (zahraniční konf.)
Tacke, S. - Krzyžánek, Vladislav - Nüsse, H. - Rosenthal, A. - Klingauf, J. - Wepf, R.A. - Reichelt, R.
A versatile high-vacuum cryo transfer system for cryo microscopy and analytics.
EMC2016. The 16th European Microscopy Congres. Proceedings. Oxford: Wiley, 2016, s. 412-413. ISBN 9783527808465.
[EMC2016. European Microscopy Congress /16./. Lyon (FR), 28.08.2016-02.09.2016]
Grant CEP: GA ČR(CZ) GA14-20012S
Institucionální podpora: RVO:68081731
Klíčová slova: cryo-EM * High-Vacuum cryo Transfer
Kód oboru RIV: JA - Elektronika a optoelektronika, elektrotechnika
http://onlinelibrary.wiley.com/doi/10.1002/9783527808465.EMC2016.6577/pdf

The conservation of the native state during sample preparation is mandatory for a correct interpretation of any micrograph. Particularly for EM, the preservation of the pristine architecture is challenging. If not imaged in situ, two different strategies can be followed to prepare hydrated samples for electron microscopy: the conventional and the cryogenic routine. Conventional preparation protocols typically rely on the chemical fixation and staining of the sample material, whereby both steps are known to induce artefacts. The second preparation routine, introduced by Moor and Dubochet, circumvents chemical artefacts. The basic principle of every cryogenic (cryo) preparation protocol is the physical immobilization of the sample in a frozen-hydrated solid state, by vitrifying the sample within milliseconds. Due to the excellent structural preservation cryo imaging techniques have gained increasing popularity.
Trvalý link: http://hdl.handle.net/11104/0263969