A Versatile High-Vacuum Cryo-transfer System for Cryo-microscopy and Analytics

0465045 - ÚPT 2017 RIV US eng J - Článek v odborném periodiku
Tacke, S. - Krzyžánek, Vladislav - Nüsse, H. - Wepf, R.A. - Klingauf, J. - Reichelt, R.
A Versatile High-Vacuum Cryo-transfer System for Cryo-microscopy and Analytics.
Biophysical Journal. Roč. 110, č. 4 (2016), s. 758-765. ISSN 0006-3495. E-ISSN 1542-0086
Grant CEP: GA ČR(CZ) GA14-20012S
Institucionální podpora: RVO:68081731
Klíčová slova: transmission electron microscopy * cryoelectron tomography * cells
Kód oboru RIV: JA - Elektronika a optoelektronika, elektrotechnika
Impakt faktor: 3.656, rok: 2016

Cryogenic microscopy methods have gained increasing popularity, as they offer an unaltered view on the architecture of biological specimens. As a prerequisite, samples must be handled under cryogenic conditions below their recrystallization temperature, and contamination during sample transfer and handling must be prevented. We present a high-vacuum cryo-transfer system that streamlines the entire handling of frozen-hydrated samples from the vitrification process to low temperature imaging for scanning transmission electron microscopy and transmission electron microscopy. A template for cryo-electron microscopy and multimodal cryo-imaging approaches with numerous sample transfer steps is presented.
Trvalý link: http://hdl.handle.net/11104/0263768