Concomitant use of polarization and positive phase contrast microscopy for the study of microbial cells

0464007 - MBÚ 2017 RIV CZ eng J - Článek v odborném periodiku
Žižka, Zdeněk - Gabriel, Jiří
Concomitant use of polarization and positive phase contrast microscopy for the study of microbial cells.
Folia Microbiologica. Roč. 60, č. 6 (2015), s. 545-550. ISSN 0015-5632. E-ISSN 1874-9356
Institucionální podpora: RVO:61388971
Klíčová slova: polarization microscopy * microbial cells * positive phase contrast
Kód oboru RIV: EE - Mikrobiologie, virologie
Impakt faktor: 1.335, rok: 2015

Polarization and positive phase contrast microscope were concomitantly used in the study of the internal structure of microbial cells. Positive phase contrast allowed us to view even the fine cell structure with a refractive index approaching that of the surrounding environment, e.g., the cytoplasm, and transferred the invisible phase image to a visible amplitude image. With polarization microscopy, crossed polarizing filters together with compensators and a rotary stage showed the birefringence of different cell structures. Material containing algae was collected in ponds in SA1/2koA (TM) ice and Zbeno villages (KA (TM) ivoklat region). The objects were studied in laboratory microscopes LOMO MIN-8 Sankt Petersburg and Polmi A Carl Zeiss Jena fitted with special optics for positive phase contrast, polarizers, analyzers, compensators, rotary stages, and digital SLR camera Nikon D 70 for image capture. Anisotropic granules were found in the cells of flagellates of the order Euglenales, in green algae of the orders Chlorococcales and Chlorellales, and in desmid algae of the order Desmidiales.
Trvalý link: http://hdl.handle.net/11104/0263354