Effect of O-methyl-.beta.-cyclodextrin-modified magnetic nanoparticles on the uptake and extracellular level of L-glutamate in brain nerve terminals

0463732 - ÚMCH 2017 RIV NL eng J - Článek v odborném periodiku
Horák, Daniel - Beneš, Milan J. - Procházková, Zuzana - Trchová, Miroslava - Borysov, A. - Pastukhov, A. - Paliienko, K. - Borisova, T.
Effect of O-methyl-.beta.-cyclodextrin-modified magnetic nanoparticles on the uptake and extracellular level of L-glutamate in brain nerve terminals.
Colloids and Surfaces B-Biointerfaces. Roč. 149, 1 January (2017), s. 64-71. ISSN 0927-7765. E-ISSN 1873-4367
Grant CEP: GA ČR(CZ) GC16-01128J; GA MŠMT(CZ) LQ1604; GA MŠMT(CZ) ED1.1.00/02.0109
Institucionální podpora: RVO:61389013
Klíčová slova: glutamate * cholesterol * O-methyl-beta-cyclodextrin
Obor OECD: Polymer science
Impakt faktor: 3.997, rok: 2017

Changes in cholesterol concentration in the plasma membrane of presynaptic nerve terminals non-specifically modulate glutamate transport and homeostasis in the central nervous system. Reduction of the cholesterol content in isolated rat brain nerve terminals (synaptosomes) using cholesterol depleting agents decreases the glutamate uptake and increases the extracellular level of glutamate in nerve terminals. Extraction of cholesterol from the plasma membrane and its further removal from the synaptosomes by external magnetic field can be achieved by means of magnetic nanoparticles with immobilized cholesterol-depleting agent such as O-methyl-beta-cyclodextrin (MCD). A simple approach is developed for preparation of maghemite (gamma-Fe2O3) nanoparticles containing chemically bonded MCD. The method is based on preparation of a silanization agent containing MCD. It is synthesized by the reaction of triethoxy(3-isocyanatopropyl)silane with MCD. Base-catalyzed silanization of superparamagnetic gamma-Fe2O3 provides a relatively stable colloid product containing 48 mu mol of MCD g(-1). MCD-modified gamma-Fe2O3 nanoparticles decrease the initial rate of the uptake and accumulation of L-[C-14]glutamate and increase the extracellular L-[C-14]glutamate level in the preparation of nerve terminals. The effect of MCD-immobilized nanoparticles is the same as that of MCD solution; moreover, magnetic manipulation of the nanoparticles enables removal of bonded cholesterol.
Trvalý link: http://hdl.handle.net/11104/0264262